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目的:研究M受体亚型在正常成人前列腺组织中的分布状况。方法:取健康成人新鲜尸体前列腺标本17例,用免疫组织化学染色与荧光定量逆转录聚合酶链反应(Real Time RT-PCR)2种方法探讨M受体各亚型(M1~M3)在正常成人前列腺组织中的分布特征。结果:2种方法分别从蛋白水平和基因水平证实M受体亚型(M1~M3)在正常成人前列腺组织中均有分布,其中以M2受体密度最高;各亚型主要分布在腺上皮周围,间质分布相对较少。结论:M受体亚型在正常成人前列腺组织中具有一定的分布规律,对发挥前列腺功能具有重要作用。
Objective: To study the distribution of M receptor subtype in normal adult prostate tissue. Methods: Seventeen specimens of healthy adult fresh cadaver specimens were obtained. Two types of M receptor subtypes (M1 ~ M3) were detected by immunohistochemical staining and real time RT-PCR. Distribution characteristics of adult prostate tissue. Results: Two methods were used to confirm the distribution of M receptor subtypes (M1 ~ M3) in normal adult prostate tissues at protein and gene level, respectively. Among them, the M2 receptor density was the highest. The subtypes were mainly distributed around the glandular epithelium , Interstitial distribution is relatively small. Conclusion: M receptor subtypes have regular distribution in normal adult prostate tissue and play an important role in the development of prostate function.