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1目的 探讨 c- fos蛋白在大鼠脑缺血再灌注脑损伤过程中的表达 ,以及川芎嗪和尼莫通对其影响。2方法 采用免疫组化法检测大鼠脑缺血再灌注不同时间内 c- fos蛋白表达的水平 ,以及川芎嗪和尼莫通干预后c- fos蛋白表达的变化。3结果 脑缺血再灌注时缺血侧脑皮质和基底核区均有 c- fos蛋白阳性表达 ,其阳性表达率随再灌注时间长短不同而不同 ,于缺血 30 min再灌注 1h时阳性表达达高峰 (34 .6 1% )。缺血 30 m in再灌注 30 m in和1h时 ,川芎嗪干预组和尼莫通干预组大鼠脑皮质和基底核区 c- fos蛋白的表达均明显下降 (χ2 =2 40 .45 6～719.96 6 ,P<0 .0 0 1)。4结论 c- fos蛋白参与大鼠脑缺血再灌注时缺血细胞损伤的发生 ,川芎嗪和尼莫通可明显下调 c- fos蛋白的表达。
Objective To investigate the expression of c-fos protein during brain injury induced by cerebral ischemia-reperfusion in rats and the effects of ligustrazine and nimotop. 2 Methods Immunohistochemistry was used to detect the level of c-fos protein expression in different time after cerebral ischemia-reperfusion in rats, and the change of c-fos protein expression after intervention of ligustrazine and Nimotop. 3 Results Cerebral ischemia and reperfusion The c-fos protein expression was positive in the cerebral cortex and basal ganglia. The positive expression rate of c-fos protein varied with the time of reperfusion, and it was positively expressed at 30 min after ischemia and 1 h after reperfusion. Peak (34.6%). At 30 min in ischemia and 30 min in reperfusion, the expression of c-fos protein in the cerebral cortex and basal ganglia of rats in the tetramethylpyrazine intervention group and Nimotop intervention group was significantly decreased (χ2 =2 40 .45 6～ 719.96 6, P<0. 0 0 1). 4 Conclusions c-fos protein is involved in the ischemic cell injury induced by cerebral ischemia-reperfusion in rats. Tetramethylpyrazine and nimotop can significantly down-regulate the expression of c-fos protein.