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目的 :探讨外源性生长抑素类似物施他宁对胰腺癌细胞的生长抑制和凋亡作用。方法 :采用MTT和细胞流式仪观测施他宁对胰腺癌细胞的生长抑制和凋亡的作用 ,并分析其对胰腺癌移植瘤的生长影响。结果 :发现PC - 3细胞在高浓度施他宁的作用下生长缓慢 ,与低浓度比较抑制率有明显差异 (P <0 .0 5) ,并与施他宁浓度呈依赖关系。施他宁对胰腺癌细胞凋亡有促进作用 ,凋亡率为 6.31 % ,而非治疗组的细胞凋亡率为 0 .48% ,两组间有非常显著差异(P <0 .0 5)。在移植瘤中 ,施他宁治疗组在肿瘤重量和肿瘤生长速度上均小于对照组 ,其差异有统计学意义 (P <0 .0 5)。结论 :施他宁对胰腺癌生长起抑制作用和促进凋亡作用。有潜在的临床应用价值
Objective: To investigate the effect of exogenous somatostatin analogue statyn on pancreatic cancer cell growth inhibition and apoptosis. Methods: MTT and cell flow cytometer were used to observe the effects of shitainin on the growth inhibition and apoptosis of pancreatic cancer cells, and their effects on the growth of pancreatic cancer xenografts were analyzed. Results: PC - 3 cells were found to grow slowly under the action of high concentration of sitatine, and there was a significant difference (P <0. 05) between the inhibition rate and the concentration of the same. Shituning promoted the apoptosis of pancreatic cancer cells, with the apoptosis rate of 6.31%, while the apoptosis rate of non-treatment group was 0.48%. There was a significant difference between the two groups (P <0.05) . In the xenograft tumor, the weight and tumor growth rate of the patients in the systemic treatment group were smaller than those in the control group, with significant difference (P <0.05). Conclusion: Sitatin can inhibit the growth of pancreatic cancer and promote apoptosis. Potential clinical application value