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以大肠杆菌ＲＹ＿（１３），Ｋ＿（１２１１００）为酶源菌株，采用聚乙烯亚胺分离纯化了限制性内切酶ＥｃｏＲＩ，并对λ－ＤＮＡ有５个专一的切割位点，将λ－ＤＮＡ切割成６个分子量不同的片段，经琼脂糖凝胶电泳后，可得６个迁移位置不同的条带。这些特性使它特别适合于作为ＤＮＡ结构功能等方面研究的工具酶。在遗传工程研究中，限制酶更起了决定性作用。
The restriction endonuclease EcoRI was isolated and purified from E. coli RY_ (13) and K_ (121100) by using polyethyleneimine and 5 specific cutting sites on λ-DNA. The λ- The DNA was cut into six fragments of different molecular weights. After agarose gel electrophoresis, six different bands could be obtained. These characteristics make it particularly suitable as a tool for the study of DNA structure and function. Restriction enzymes have also played a decisive role in genetic engineering research.