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研究超低温冷冻保存(-196℃)对日本鳗鲡精子内总ATP酶、肌酸激酶(CK)、琥珀酸脱氢酶(SDH)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)等酶活性的影响。运用试剂盒分别测定了冷冻前后日本鳗鲡精子内酶活性的变化。结果表明,经过超低温冷冻保存后,日本鳗鲡精子的活力下降,精子内GR活性显著升高(P<0.05),酶活性从冻前的358.52±45.65 U/L上升到646.30±70.30 U/L;其它几种酶的活性均显著下降(P<0.05),总ATP酶、CK和SDH的活性分别从冻前的3.14±0.61 U/ml、17.10±3.51 U/ml和32±5.94 U/ml下降到1.83±0.43 U/ml、7.33±1.74 U/ml和21±1.41 U/ml,LDH、SOD和CAT活性分别从冻前的2 266.67±313.25 U/L、220.47±32.94 U/ml和48.51±5.94U/ml下降到1 195.91±198.51 U/L、84.16±22.11 U/ml和21.8±4.14 U/ml。超低温冷冻对日本鳗鲡精子酶活性和精子活力均有较大影响。
The effects of cryopreservation (-196 ℃) on total ATPase, creatine kinase (CK), succinate dehydrogenase (SDH), lactate dehydrogenase (LDH), superoxide dismutase (SOD) , Catalase (CAT), glutathione reductase (GR) and other enzyme activities. The changes of enzyme activities in sperm of Japanese eel before and after freezing were measured respectively by using the kit. The results showed that after ultra-low temperature cryopreservation, the sperm motility of Japanese eel sperm decreased, the activity of GR in sperm increased significantly (P <0.05), and the enzyme activity increased from 358.52 ± 45.65 U / L before freezing to 646.30 ± 70.30 U / L. The activity of other enzymes decreased significantly (P <0.05). The activities of total ATPase, CK and SDH decreased from 3.14 ± 0.61 U / ml, 17.10 ± 3.51 U / ml and 32 ± 5.94 U / ml before freezing To 1.83 ± 0.43 U / ml, 7.33 ± 1.74 U / ml and 21 ± 1.41 U / ml, LDH, SOD and CAT activities increased from 2 266.67 ± 313.25 U / L, 220.47 ± 32.94 U / ml and 48.51 ± 5.94 U / ml to 1 195.91 ± 198.51 U / L, 84.16 ± 22.11 U / ml and 21.8 ± 4.14 U / ml. Cryopreservation had a significant effect on sperm enzyme activity and sperm motility in Japanese eel.