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采用聚丙烯酰胺凝胶电泳技术,研究了17个苜蓿品种在人工接种和不接种病原尖孢镰刀菌的情况下过氧化物酶同工酶的变化,并探讨了同工酶与品种抗病性之间的相关性。结果表明:不论接菌与否,过氧化物同工酶的谱带数、最大迁移率以及同工酶活性在苜蓿品种幼苗根部均存在较大差异,且接菌处理后与对照间也存在明显不同;从同工酶的酶带数和最大迁移率来看,与品种对根和根颈腐烂病抗性间的相关性均未达到显著水平,而且在抗病品种和感病品种之间也没有规律性变化;但从同工酶活性的变化上分析发现,幼苗根部接菌后同工酶活性有降低的趋势。
Polyacrylamide gel electrophoresis was used to study the changes of peroxidase isozymes in 17 alfalfa cultivars with and without inoculation of Fusarium oxysporum, The correlation between. The results showed that there was a big difference in the number of bands, the maximum mobility and the isoenzyme activity of alfalfa isolates regardless of inoculation or inoculation, and there were obvious differences between the treatments after inoculation and the control Different from the isoenzyme enzyme band number and the maximum mobility point of view, and the varieties of root and root rot rot resistance were not significantly correlated, but also between the resistant varieties and susceptible varieties No regular changes; but isoenzyme activity changes from the analysis found that the seedling roots after isozyme activity decreased.