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为了研究花生属异源多倍体进化过程中的生理特性遗传变化规律,以花生区组栽野种间杂种F1、早期多倍体世代(S0~S3)及其亲本为材料,分析植株叶片中的脯氨酸、丙二醛、可溶性糖和叶绿素含量以及过氧化物酶活性等抗病抗逆相关生理生化指标的变化特征。结果表明,杂种F1代各项生理指标都高于亲本,表现出明显的杂种优势;染色体加倍后的S0~S1代植株叶片中的POD活性、脯氨酸含量和叶绿素含量均高于F1代,S1~S3代各项生理指标伴随着自交代数的增加而逐渐降低,但仍高于母本栽培种,说明染色体加倍后的多倍体植株可能具有更强的抗病、抗旱等抗逆和环境适应能力。
In order to study the genetic variation of physiological characteristics of allotetraploid peanut genotypes, the interspecific hybrid F1, early polyploid generation (S0 ~ S3) Proline, malondialdehyde, soluble sugar and chlorophyll content and peroxidase activity and other physiological and biochemical indexes related to disease resistance and stress resistance. The results showed that all physiological indexes of hybrid F1 were higher than that of their parents and showed obvious heterosis. POD activity, proline content and chlorophyll content in S0 ~ S1 generation plants after chromosome doubling were higher than those in F1 generation, The physiological indexes of S1 ~ S3 decreased gradually with the increase of selfed generations, but were still higher than that of female parent, indicating that the polyploid plants after doubled chromosomes may have more resistance to disease and drought Environmental adaptability.