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在光诱导条件下,采用HE染色法和四甲基偶氮唑蓝比色法(MTT法),研究了纳米TiO_2对Bel-7402人体肝癌细胞的杀伤作用,考察了在不同纳米TiO_2浓度、不同光照时间下纳米TiO_2的抑瘤效果,并且对抑瘤机制进行了探讨．结果发现：在光诱导条件下,适宜的了TiO_2浓度具有较高的抑瘤率,同时抑瘤过程表现出类似一级反应的动力学规律；在光诱导条件下,纳米TiO_2产生的活性氧组分与癌细胞膜内外的生物大分子反应,引起广泛的细胞结构破坏；造成癌细胞内Ca~(2+)离子稳态失去平衡；引发细胞微管相关蛋白2(MAP-2)表达的变化,促进微管发生重组,从而导致细胞凋亡和坏死．
Under light-induced conditions, the killing effect of nano-TiO 2 on Bel-7402 human hepatoma cells was studied by HE staining and MTT method. The effects of nano TiO 2 at different concentrations of TiO 2 Anti-tumor effect of nano-TiO2 under light irradiation time, and the anti-tumor mechanism was discussed. The results showed that under the light-induced conditions, the suitable TiO 2 concentration had a higher inhibition rate, and the tumor-inhibiting process showed a similar kinetic law as the first-order reaction. Under light-induced conditions, the active oxygen species Which leads to the reaction of biological macromolecules inside and outside the membrane of cancer cells, causing extensive destruction of cell structure; causing the homeostasis of Ca 2+ ions in cancer cells to lose balance; initiating the change of the expression of microtubule-associated protein 2 (MAP-2) Promote microtubule recombination, leading to apoptosis and necrosis.