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目的对血清HBV标志物阴性肾炎患者肾组织进行HBV-DNA的检测,为临床诊疗提供理论依据。方法应用原位杂交(ISH)技术检测37份血清HBVAg阴性和18份血清HBVAg阳性的肾炎患者肾活检石蜡包埋组织切片中的HBV-DNA。结果在血清HBVAg阳性的18例标本中HBV-DNA的检出率为88.9%(16/18),血清HBVAg阴性37例中检测出HBV-DNA5例(13.5%)。原位杂交显示两组检出的HBV-DNA均以肾小管上皮细胞质分布为主,与组织中HBVAg阳性颗粒的分布基本一致。结论血清HBVAg阴性的HBV相关性肾炎临床上并非少见,应给予足够的关注。HBV-DNA在肾组织中的检出,表明HBV在HBV相关性肾炎的发病机理中有着重要作用。
Objective To detect HBV-DNA in serum of patients with HBV-negative nephritis and provide a theoretical basis for clinical diagnosis and treatment. Methods In situ hybridization (ISH) was used to detect HBV-DNA in 37 paraffin-embedded tissue sections from 37 HBsAg-negative and 18 HBsAg-positive nephritis patients. Results The positive rate of HBV DNA was 88.9% (16/18) in 18 HBsAg positive and 5 (13.5%) HBV DNA in 37 HBsAg negative. In situ hybridization showed that HBV-DNA detected by both groups were mainly cytoplasmic distribution of renal tubular epithelial cells, and the distribution of HBVAg-positive particles in tissues was basically the same. Conclusion Serum HBVAg-negative HBV-associated nephritis is not uncommon and should be given enough attention. Detection of HBV-DNA in kidney tissue indicates that HBV plays an important role in the pathogenesis of HBV-associated nephritis.