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利用T-Hg(Ⅱ)-T特异性结合作用识别汞离子(Hg~(2+)),以G-四联体/卟啉类化合物NMM(N-甲基卟啉二丙酸IX)复合结构(G-四联体/NM M)作为荧光信号分子,开发了一种新型汞离子传感器。该传感器操作简单、经济实用且不需要人工修饰化学基团,而且由于G-四联体/NMM的最大发射波长为615 nm,有效避免了生物自发荧光的干扰。研究表明,在对钾离子浓度、NMM浓度等反应条件进行优化后,该传感器对汞离子的检测具有较高的选择性和灵敏性,检出限低至20 nmol/L,与世界卫生组织(WHO)所规定的饮用水中30 nmol/L汞离子标准相当,具有用于检测饮用水中汞离子的潜在实用价值。
The mercury ion (Hg 2+) was identified by T-Hg (II) -T specific binding and the G-quadruplex / porphyrin compound NMM (N-methylporphyrin dipropionate IX) Structure (G-Quadruplex / NM M) As a fluorescent signal molecule, a new type of mercury ion sensor has been developed. The sensor is simple to operate, economical and does not require chemical modification of the chemical groups. Moreover, the maximum emission wavelength of the G-quadruplex / NMM is 615 nm, which effectively avoids the biological autofluorescence interference. The results show that the sensor has high selectivity and sensitivity for mercury ion detection after optimization of reaction conditions, such as potassium ion concentration and NMM concentration. The detection limit is as low as 20 nmol / L, which is close to that of the World Health Organization WHO) provides a comparable standard of 30 nmol / L mercury in drinking water, with the potential utility to detect mercury ions in drinking water.