Objective:　To investigate the apoptosis rules of the astrocytes and oligodendrocytes induced by Ca2+ reperfusion. rn　　Methods:　The apoptosis of purified cultured astrocytes and oligodendrocytes induced by Ca2+ reperfusion and the relationship between the development of the cell apoptosis and post-reperfusion time was observed. rn　　Results:　Both the astrocytes and oligodendrocytes were obviously in a time-dependent fashion, and the apoptosis ratios of the oligodendrocytes (39.73%±4.16%) were higher than the astrocytes (19.64%±4.67%) 24 hours after Ca2+ reperfusion. The TUNEL positive cells were 13.6±1.82 and 21.4±1.95 at every visual field of astrocytes and oligodendrocytes respectively 24 hours after Ca2+ reperfusion.rn　　Conclusions:　The astrocytes and oligodendrocytes are similar with the development rules on apoptosis and have different susceptiveness to the situation.