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目的:该文对从黑龙江省密山、吉林省珲春及辽宁省宽甸三个地区分离到的恙虫病立克次体每株的生物学及免疫学性质进行了比较分析.方法:采用间接免疫荧光法、小鼠保护试验以及SDS-聚丙烯胺电泳法,分别测定分离株的血清类型、毒力和蛋白图谱.结果:三个地区的分离株除二株外均能引起小白鼠典型的发病过程.密山和珲春分离株均以Gilliam型为主;宽甸分离株则以Karp型为主.毒力以密山分离株最强(LD50=6.5-8.1);珲春分离株次之(LD50=4.6-4.8);宽甸分离株较弱(LD50=2.0-3.2).结论:东北地区恙虫病立克次体分离株存在着3种血清型,其毒力由北向南逐渐减弱的趋势.三个地区的分离株都具有较好的免疫力,SDS-聚丙烯酰胺电泳所显示的各分离株图谱未见差异.“,”To make an analysis and comparison of the biological and immunoloical properties of the isolated Rt strains from Mishan in Heilongjiang Province,Hunchun in Jinn Province and Kuandian in Liaoning Province of Northeast China. METHOD:Use the method of indirect immunofluorescence,mice cross protection test and SDS -PAGE to determine the serum type,the toxicity and the protein atlas of the isolated strains. RESULTS:All the sixteen isolated Rt, strains from the three districts had caused the mice to fall ill except two. The isolated strains from Mishan and Hunchun are mostly Gilliam type-the ones from Kuandian are mostly Karp type. The toxicity of the strains are LD50=10-6.5~-8.1(Mishan)、LD50=10-4.6~-4.8 (Hunchun)、LD50=10-2.0~-3.2 (Kuandian), sepa ratly. There existed relatively strong protective function between the isolated strains and Karp standard strains.The protection index is 3.00~4. 00. CONCLUSION: There are three serum types among the isolate Rt. srains in Northeast China. The toxicity of the isolated strains shown a weakening tendency from the North to the South.The isolated strains posessed relatively strong immune ability. There appeared no difference in SDS-PAGE atlas.