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目的:探讨活血化痰方(十味肝脂康胶囊)对脂肪肝大鼠的防治作用,寻找中医药治疗脂肪肝的有效方法和最佳组方,并为其更广泛的临床应用提供实验依据。方法:取Wistar大鼠60只以普通饲料适应性喂养1周后,按体重随机分为6组,即正常组10只,模型组10只,十味肝脂康高剂量组10只,中剂量组10只,低剂量组10只,东宝肝泰组10只。依文献复制大鼠脂肪肝模型,同时给实验动物灌服试验药物和对照药物。8周后检测各组肝组织中TG、TC及FFA含量;光镜下观察病理形态变化,透射电镜观察肝组织超微结构变化。结果:①与模型组比较,各用药组脂肪肝大鼠体重、肝指数及肝匀浆TG、TC、FFA均有不同程度的下降,十味肝脂康胶囊高、中、低剂量组的药效作用强度有一定的剂量依赖关系。其中以十味肝脂康中剂量组疗效最为显著(P<0.01或P<0.05)。②与东宝肝泰组比较,中剂量组大多数指标均有不同程度的下降(P<0.01或P<0.05),明显优于东宝肝泰组。结论:活血化痰方(十味肝脂康胶囊)防治脂肪肝的机制在于调整脂质代谢,减少脂质在肝脏中的沉积;促进肝内微循环,修复和保护肝细胞质膜、线粒体膜,从而改善肝组织病理形态结构变化,保护肝细胞,促使肝细胞功能恢复。
Objective: To explore the preventive and therapeutic effects of Huoxuehuayu Decoction (Shiwei Ganzhikang Capsule) on fatty liver rats, to find an effective method and the best prescription for the treatment of fatty liver by Chinese medicine, and to provide experimental basis for its more extensive clinical application. . METHODS: Sixty Wistar rats were fed with normal diet for 1 week. They were randomly divided into 6 groups according to their body weight. There were 10 normal rats, 10 model rats, and 10 high-dose Shiwei Ganzhikang groups. There were 10 rats in the group, 10 in the low-dose group and 10 in the Dongbaogantai group. Rat fatty liver model was reproduced according to the literature, and experimental animals and control drugs were given to experimental animals at the same time. After 8 weeks, the contents of TG, TC and FFA in liver tissue were detected. The pathological changes were observed under light microscope. The ultrastructural changes of liver tissue were observed under transmission electron microscope. Results: 1Compared with the model group, the body weight, liver index and liver homogenates of TG, TC, and FFA in the fatty liver of each drug group decreased to varying degrees. The drugs in the high-, middle-, and low-dose groups of Shiwei Ganzhikang capsules The effect intensity has a certain dose-dependent relationship. Among them, the effect of Shiwei Ganzhikang middle dose group was the most significant (P<0.01 or P<0.05). 2 Compared with Dongbao Gantai Group, most of the indicators in the middle-dose group decreased to different degrees (P<0.01 or P<0.05), which was significantly better than Dongbao Gantai Group. Conclusion: The mechanism of prevention and treatment of fatty liver by Huoxue Huayu Decoction (Shiwei Ganzhikang Capsule) is to regulate lipid metabolism, reduce the deposition of lipids in the liver, promote intrahepatic microcirculation, and repair and protect hepatocyte plasma membrane and mitochondrial membrane. In order to improve the pathological changes of liver tissue, protect liver cells and promote liver cell function recovery.