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目的:HPLC法定量分析不同产地、不同生长年限的刺五加药材中异嗪皮啶。方法:采用高效液相色谱法,色谱柱:Kromasil C18分析柱(200mm×4.6mm,5μm);流动相:乙腈-0.1%磷酸(20∶80);流速:1mL·min-1;检测波长:343nm;柱温:30℃;进样量20μL。结果:异嗪皮啶进样量在0.0171～0.3024μg范围内呈现良好的线性关系,平均回收率为99.4%,RSD为2.9%(n=9)。铁西产刺五加根中异嗪皮啶含量2年生最高(110.15μg.g-1),茎中1年生最高;么河产不同生长年限刺五加中含量差别不大;根中异嗪皮啶的含量明显高于茎;铁西产刺五加中异嗪皮啶含量比么河产高。结论:本法操作简便、准确,专属性强,适用于刺五加中异嗪皮啶含量测定,该研究可为刺五加最佳产地、最适宜采收年限及最佳药用部位的确定提供参考。
OBJECTIVE: To quantify isoindoline in Acanthopanax senticosus by different HPLC methods. Methods: High performance liquid chromatography (HPLC) was used. The analytical column was Kromasil C18 (200mm × 4.6mm, 5μm). The mobile phase was acetonitrile-0.1% phosphoric acid 343nm; column temperature: 30 ℃; injection volume 20μL. Results: The isoxapiram sample showed a good linearity in the range of 0.0171-0.3024 μg with an average recovery of 99.4% and RSD of 2.9% (n = 9). The content of mid-isoxabepilone was the highest in the two-year-olds (110.15μg.g-1) in Tiexie, and the one-year-old in the stems was the highest. The content of picoline was significantly higher than that of the stem. The content of iso-puronene in Acanthopanax senticosus was higher than that in Hexi. Conclusion: The method is simple, accurate and specific, and is suitable for the determination of isoquinolide in Acanthopanax senticosus. This study could be used to determine the optimum producing area, the optimum harvesting time and the best medicinal site for acanthopanax senticosus for reference.