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神经功能结构的建立和维持是学习与记忆等高级功能的基础.因神经网络的结构错综复杂,节点行为多变且受多种因素的影响,目前其拓扑结构的形成和维持机制尚不清楚.基于多电极阵列系统,本研究连续记录了体外长时间培养的海马神经元网络的自发放电活动;利用不同电极放电序列之间的互相关系数映射功能连接强度,构建网络拓扑结构图,分析其发育特性.对培养1～18周的3个样本的分析结果显示,网络拓扑结构的形成和维持具有自组织和自解离特性:网络连接建立期(1～3周),邻近节点之间建立连接,形成局域微网络;随着网络的发育,逐步形成多节点的复杂功能结构;发育成熟期(12周后),功能连接开始消退,部分节点脱离网络,网络聚集程度增加.不同发育时期网络各节点连接强度的分布均符合幂率分布,表明无外界输入的培养神经元网络是具有无尺度特征的复杂网络.本研究有助于复杂神经网络结构的形成及维持机制分析,为研究神经发育和神经系统信息处理机制提供新的视野.
The establishment and maintenance of neural functional structures are the basis of advanced functions such as learning and memory.Because the structure of neural networks is complex and the behavior of nodes is changing and influenced by many factors, the mechanism of forming and maintaining topological structure is not clear at present.Based on Multi-electrode array system, this study continuously recorded long-term in vitro cultured hippocampal neuronal network spontaneous discharge activity; the use of different electrode discharge sequence correlation coefficient between the mapping function of the connection strength, the network topology structure diagram, analysis of its developmental characteristics The results of three samples from 1 to 18 weeks showed that the formation and maintenance of network topology had the characteristics of self-organization and self-dissociation: the network connection establishment period (1-3 weeks), the connection between neighboring nodes, Forming a local micro-network; With the development of the network, gradually forming a complex multi-node functional structure; mature period (after 12 weeks), functional connectivity began to subside, some nodes off the network, network aggregation increased.Different developmental networks The distribution of node connection strength is in accordance with the power distribution, indicating that the neural network with no outside input is a complex network with no scale features This study contributes to the formation and maintenance of complex neural mechanisms of network structure analysis, to provide a new perspective for the study of neural development and neural mechanisms of information processing systems.