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目的:观察右侧海马(HPC)微量注射印防己毒素(PTX)诱导HPC癫痫电网络重建过程中HPC-体循环动脉血压调节网络的形成。方法:将PTX(7.2μg)微量注射到大鼠右侧HPC诱发HPC癫痫,四通道同步记录左侧深部电图、单个HPC细胞外单位放电、左侧股动脉血压和标准II导联心电图。结果:将PTX微量注射到右侧HPC后可以引起以下效应:①对侧HPC神经元长时程爆发式单位放电与单位后放电,并具有相似的脉冲间隔(interspikeintervals,ISI)点分布;②延迟对侧HPC神经元爆发式单位放电与相对应的股动脉血压下降发生的时间关系;③出现复合式的对侧HPC神经元爆发式单位放电或单位后放电和股动脉血压下降耦合;④具有相似点分布特征的对侧HPC网络波峰间隔(interpeak intervals,IPI)和单个神经元ISI共同参与了HPC-体循环动脉血压调节网络的构成。结论:将PTX微量注射到右侧HPC可以在诱导对侧HPC癫痫网络形成的同时通过特征性的瞬时编码形式调制HPC-体循环动脉血压调节网络的功能活动。
OBJECTIVE: To observe the formation of HPC-circulatory arterial pressure regulating network in the process of HPC epileptic electrical network induced by microinjection of picrotoxin (PTX) into right hippocampus (HPC). Methods: PTX (7.2μg) was injected into right HPC induced HPC epilepsy in rats by microinjection. The left deep electrograms, single extracellular HPC, left femoral arterial pressure and standard II lead electrocardiogram were simultaneously recorded in four channels. RESULTS: Microinjection of PTX into the right HPC resulted in the following effects: (1) The contralateral HPC neurons had long-range explosive discharge and unit discharge with similar distribution of interspikeintervals (ISI); ② delay The contralateral HPC neuron burst unit discharge and the corresponding decline in the frequency of femoral artery blood pressure occurred; ③ appeared contralateral HPC neurons burst unit discharge or unit discharge and femoral artery blood pressure decoupling; ④ have similar The contralateral HPC network interpeak intervals (IPI) and single neuron ISI participate in the formation of HPC-circulatory arterial pressure regulating network. CONCLUSION: Microinjection of PTX into the right HPC modulates the functional activity of the HPC-systolic arterial pressure regulation network via a characteristic transient encoding form while inducing contralateral HPC epilepsy network formation.