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使用超高效液相色谱-串联质谱联用技术(UPLC-MS/MS)建立了人尿中5种利尿剂的检出方法。将尿样用同体积的5%乙酸铅水溶液沉淀蛋白质并离心后,将上清液用多反应监测(MRM)模式进行定性和定量分析。上述利尿剂1~5的检测限(LOD)在0.5~2 ng/m L之间;线性范围分别为3.0~700 ng/m L,1.5~1000 ng/m L,3.0~4000 ng/m L,6.0~5000 ng/m L和6.0~5000 ng/m L,线性良好(R2>0.99);在低、中和高3个浓度水平的日内和日间RSD均小于15%。
The detection methods of five diuretics in human urine were established by using UPLC-MS / MS. After the urine samples were pelleted with the same volume of 5% aqueous lead acetate and centrifuged, the supernatants were subjected to qualitative and quantitative analysis in a multiplex reaction monitoring (MRM) mode. The limits of detection (LODs) for diuretics 1-5 ranged from 0.5 to 2 ng / m L with linear ranges of 3.0 to 700 ng / m L, 1.5 to 1000 ng / m L and 3.0 to 4000 ng / m L, respectively , 6.0-5000 ng / m L and 6.0-5000 ng / m L, respectively. The linearity was good (R2> 0.99). The intra-day and inter-day RSD at low, medium and high concentrations were less than 15%.