目的　研究切割bcl 2mRNA核酶对人胆管癌细胞致瘤性的影响。方法　将合成的切割bcl 2mRNA核酶 (ribozyme ,RZ)　基因定向插入到真核细胞表达载体 pDOR neo内 ,并用重组后的载体转染人胆管癌细胞QBC93 9,再将经转染核酶基因的胆管癌细胞 5× 10 6个分别接种于 2 0只裸鼠皮下 ,观察该肿瘤细胞的致瘤情况。结果　转染切割bcl 2mRNA核酶基因的胆管癌细胞的bcl 2表达水平及裸鼠体内致瘤性 (0 / 5 )较对照组 (8/ 10和 5 / 5 )明显下降。结论　该切割bcl 2mRNA核酶能明显降低人胆管癌细胞内bcl 2的表达水平及其在裸鼠体内的致瘤性 Objective To study the effect of bcl 2 mRNA ribozyme cleavage on the tumorigenicity of human cholangiocarcinoma cells. METHODS: The synthetically digested bcl 2 mRNA ribozyme (RZ) gene was inserted into the eukaryotic expression vector pDOR neo, and the recombinant vector was transfected into the human cholangiocarcinoma cell QBC93 9, and the transfected ribozyme gene was transfected. 5 × 10 6 cholangiocarcinoma cells were inoculated subcutaneously in 20 nude mice and the tumorigenicity of the tumor cells was observed. Results The expression of bcl 2 in bile duct cancer cells transfected with bcl 2 mRNA ribozyme gene and tumorigenicity (0 / 5) in nude mice were significantly lower than those in control group (8/10 and 5/5). Conclusion The cleavage of bcl 2 mRNA ribozyme can significantly reduce the expression of bcl 2 in human cholangiocarcinoma cells and its tumorigenicity in nude mice.