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研究应用酶联免疫方法,快速检测茶叶中硫丹及硫丹硫酸酯。茶叶提取后,不净化,用PBS缓冲液稀释500倍后,进行酶联免疫方法测定,方法平均回收率为86.6%~91.1%;经SPE净化后,回收率68.7%~123%。标准曲线IC50为2μg/L,线性范围1~10μg/L。直接提取法最低检测限2mg/kg,净化法为0.06mg/kg。经气相色谱/质谱联用方法验证,灵敏度、精密度和准确度均符合快速检测要求。该法具有快速、简便、低耗、易操作等特点。
Study the application of enzyme-linked immunosorbent assay, rapid detection of endosulfan and endosulfan sulfate in tea. After the tea leaves were extracted, they were not purified. After diluted 500 times with PBS buffer, the enzyme-linked immunosorbent assay was used. The average recovery was 86.6% -91.1%. After SPE purification, the recovery was 68.7% -123%. The standard curve IC50 was 2 μg / L with a linear range of 1 to 10 μg / L. Direct extraction method minimum detection limit of 2mg / kg, purification method is 0.06mg / kg. The gas chromatography / mass spectrometry method validation, sensitivity, precision and accuracy are in line with the rapid testing requirements. The method is fast, easy, low consumption, easy to operate and so on.