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目的:研究藏药打箭菊对D-半乳糖胺(D-galactosamine,D-Gal)所致大鼠急性肝损伤的保护作用。方法:60只大鼠随机分为正常对照组、模型组、高中低剂量给药组、阳性对照组等6个组。给药组按18 g/kg、9 g/kg、4.5 g/kg剂量以打箭菊水提物灌胃;阳性对照组按50 mg/kg联苯双酯灌胃,每天1次,共7天。灌胃给药第6天下午,模型组和给药组按500 mg/kg体重腹腔注射D-Gal一次。第8天,末次给药24 h(腹腔注射36 h)后,测定血清生化指标,并测体重和肝脏重、脾重、胸腺重,求脏器系数,同时切取肝脏检测肝糖原,取肝组织作病理观察。结果:打箭菊18 g/kg、9 g/kg、4.5 g/kg剂量给药组均可显著降低急性肝损伤大鼠血清ALT、AST、ALP水平和TBA、T-Bil含量,促进血清中TP、ALB和肝组织中肝糖原的增加,且中低剂量给药组对肝重量增大和胸腺萎缩有抑制作用。病理检查结果也显示有明显的保肝作用。结论:打箭菊对D-Gal所致大鼠急性肝损伤有保护作用,其作用机制与多环节有关。
Objective: To study the protective effect of Tibetan medicine Chrysanthemum on acute liver injury induced by D-galactosamine (D-Gal) in rats. Methods: Sixty rats were randomly divided into six groups: normal control group, model group, high school and low dose group and positive control group. The rats in the treatment group were given gavage at a dose of 18 g / kg, 9 g / kg and 4.5 g / kg respectively. The positive control group was given gavage at 50 mg / kg once daily for 7 days . On the 6th day after gavage administration, D-Gal was injected intraperitoneally once a day at 500 mg / kg body weight in the model group and the administration group. On the 8th day, after the last administration for 24 hours (intraperitoneal injection for 36 hours), the serum biochemical indexes were measured, and the body weight, liver weight, spleen weight, thymus weight and organ coefficient were measured. Tissue for pathological observation. Results: The contents of ALT, AST, ALP, TBA and T-Bil in serum of rats with acute hepatic injury were significantly decreased by the doses of 18g / kg, 9g / kg and 4.5g / TP, ALB and hepatic glycogen increased, and low and middle dose groups had an inhibitory effect on liver weight gain and thymus atrophy. Pathological examination results also showed significant hepatoprotective effect. Conclusion: Daocheng chrysanthemum has the protective effect on D-Gal-induced acute liver injury in rats, and its mechanism is related to multiple links.