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为评价甜高粱资源对混合盐碱胁迫的耐受性,挖掘耐混合盐碱胁迫甜高粱资源,并筛选适合甜高粱芽期和苗期耐混合盐碱胁迫的鉴定指标,本研究设置4个不同浓度混合盐碱液(T1、T2、T3和T4,对应的Na+浓度分别为50、100、200、300mmol/L;对应的p H分别为7.12、8.01、8.80、10.07)对5个甜高粱品种进行胁迫处理,测定了甜高粱的相对发芽率、幼苗茎叶鲜重、叶片相对电导率、叶片脯氨酸含量、叶片过氧化物歧化酶(SOD)活性等指标,采用模糊数学隶属函数法和灰色关联分析法评价各材料耐盐性的强弱。结果显示,随着胁迫强度的增加,所有材料的相对发芽率和茎叶鲜重均呈现下降趋势,相对电导率和脯氨酸含量均呈现上升趋势,而SOD活性则表现出先升高后降低的趋势,在T3处理SOD活性达到最高值。依据T3处理的各项数据进行灰色关联分析,结果显示,5个测定指标与综合抗盐碱指数的关联度大小排序依次为脯氨酸含量(0.95)、茎叶鲜重(0.93)、相对电导率(0.92)、SOD活性(0.91)和相对发芽率(0.74)。5个材料耐盐碱适应能力强弱按隶属函数法的排序为雅津106号>雅津4589号>雅津2号>雅津4184号>雅津10号,按灰色关联分析法的排序为雅津2号>雅津106号>雅津4589号>雅津4184号>雅津10号。以上结果表明,5份甜高粱种质对混合盐碱胁迫的耐受性存在显著差异,茎叶鲜重、脯氨酸含量、相对电导率和SOD活性是评价甜高粱耐盐碱胁迫性的关键指标,雅津106号的抗盐碱能力最强,雅津10号的抗盐碱能力最弱。
To evaluate the tolerance of sweet sorghum resources to mixed saline-alkali stress, digging sweet sorghum resources tolerant to mixed-saline-alkali stress and screening the identification indexes suitable for tolerance to mixed saline-alkali stress in sweet and sorghum at bud and seedling stage, four different (T1, T2, T3 and T4, the corresponding Na + concentrations were 50, 100, 200 and 300 mmol / L respectively; corresponding p H values were 7.12, 8.01, 8.80 and 10.07, respectively) for 5 sweet sorghum cultivars The relative germination rate of sweet sorghum, the fresh weight of stems and leaves, the relative conductivity of leaves, the content of proline in leaves and the activity of superoxide dismutase (SOD) in leaves were measured. The fuzzy mathematics membership function method and Gray relational analysis method to evaluate the strength of salt tolerance of various materials. The results showed that with the increase of stress intensity, the relative germination rate and fresh weight of stems and leaves of all materials showed a downward trend, the relative conductivity and proline content showed an upward trend, while the SOD activity showed an increase and then decreased Trend, the SOD activity reached the highest value at T3. Based on the gray relational analysis of each data processed by T3, the results showed that the correlation degree between the five indexes and the comprehensive salt-saline-alkali index was proline content (0.95), fresh weight of stems and leaves (0.93), relative conductance Rate (0.92), SOD activity (0.91) and relative germination rate (0.74). The ability of five materials to adapt to salt and alkali is strong, and according to the membership function, the order is Ajinjin 106> Ajinjin 4589> Ajinjin 2> Ajinjin 4184> Ajinjin 10, according to the gray relational analysis Ajinjin 2> Ajinjin 106> Ajinjin 4589> Ajinjin 4184> Ajinjin 10. The above results showed that the tolerance of five sweet sorghum germplasms to mixed saline-alkali stress was significantly different. The fresh weight, proline content, relative conductivity and SOD activity of sweet sorghum were the key to evaluate the salt-alkali stress tolerance of sweet sorghum Indicators, Ajinjin 106, the ability to resist salt and strongest, Ajinjin 10, the weakest resistance to salt.