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目的:研究高氧液预处理对兔心肌缺血再灌注损伤的影响。方法:雄性新西兰白兔32只,随机分为4组(n=8),结扎-开放冠状动脉左前降支(LAD)建立心肌缺血再灌注模型。假手术组(Sham组)只穿线环绕LAD不结扎;吸氧组(OX组)结扎前30 min经鼻吸纯氧2L/min;在结扎LAD前30 min分别静脉注射HO 10 ml/kg(HO1组)、20 ml/kg(HO2组)。于结扎LAD前即刻(T0,基础值)、开放LAD前即刻(T1)、再灌注60 min(T2)及再灌注120 min(T3)时记录HR和MAP,于T3时抽取动脉血样3 ml,测定血清肌酸激酶(CK)、肌钙蛋白I(cTNI)的活性和IL-6和TNF-α的浓度,并测定心肌梗死范围。结果:I/S组与T0时比较,T 1-3时各组HR、MAP进行性下降(P<0.05);三组间HR、MAP比较差异无统计学意义(P>0.05)。与Sham组比较,I/S组血清CK、cTNI、IL-6和TNF-α含量明显升高(P 0.01);与OX组比较,HO2组上述酶及炎症因子浓度显著下降(P<0.01),心肌梗死范围减小(P<0.05)。结论:高氧液预处理可减轻兔心肌缺血再灌注损伤,机制可能与其抑制炎性反应有关。
Objective: To investigate the effect of hyperoxic liquid preconditioning on myocardial ischemia-reperfusion injury in rabbits. Methods: Thirty-two male New Zealand white rabbits were randomly divided into 4 groups (n = 8). The models of myocardial ischemia-reperfusion were established by ligating the left anterior descending coronary artery (LAD). Sham group was only lined around the LAD without ligation; oxygen group (OX group) 30min before ligation of nasal oxygen inhalation 2L / min; 30min before ligation LAD intravenous injection of HO 10ml / kg (HO1 Group), 20 ml / kg (HO2 group). HR and MAP were recorded immediately before LAD (T0, baseline), immediately before opening LAD (T1), 60 min (T2) and 120 min (T3) after reperfusion. 3 ml arterial blood samples were taken at T3, Serum creatine kinase (CK), troponin I (cTNI) activity and concentrations of IL-6 and TNF-α were measured and myocardial infarct size was measured. Results: Compared with T0 at I / S, the HR and MAP in each group decreased progressively at T1-3 (P <0.05). There was no significant difference between the three groups in HR and MAP (P> 0.05). Compared with Sham group, the levels of CK, cTNI, IL-6 and TNF-αwere significantly increased in I / S group (P <0.01) , Myocardial infarction range decreased (P <0.05). Conclusion: Hyperoxic liquid preconditioning can attenuate myocardial ischemia-reperfusion injury in rabbits, which may be related to the inhibition of inflammatory reaction.