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目的探讨肺炎支原体肺炎(MPP)患儿血清IL-27水平变化及其在MPP发病机制中的意义。方法以15名健康儿童为正常对照组,对36例MPP患儿和35例非肺炎支原体肺炎(NMPP)患儿的急性期外周血IFN-γ、IL-4和IL-27水平进行测定。血清IFN-γ、IL-4和IL-27水平采用双抗体夹心ELISA法进行检测。结果 MPP组、NMPP组急性期及对照组血清IFN-γ水平分别为(173.1±29.2)pg/ml、(192.2±36.4)pg/ml、(152.3±28.2)pg/ml;血清IL-4水平分别为(124.8±34.7)pg/m、(100.4±31.9)pg/ml、(80.0±24.7)pg/ml。MPP组、NMPP组血清IFN-γ、IL-4水平均较对照组升高(P<0.05,P<0.01),但MPP组IFN-γ/IL-4比值降低,而NMPP组则无明显变化。MPP组、NMPP组、对照组血清IL-27水平分别为(24.24±9.00)pg/ml、(29.76±10.18)pg/ml、(18.03±7.58)pg/ml,MPP组高于对照组(P<0.05),NMPP组高于MPP组(P<0.05)。MPP组及NMPP组患儿血清IL-27与IFN-γ水平呈正相关(r=0.849,P<0.01),血清IL-27水平与IFN-γ/IL-4比值呈正相关(r=0.884,P<0.01)。结论 MPP患儿急性期IL-27应答不足,Th1/Th2应答失衡,在MPP的免疫病理损伤与肺外并发症发生中可能有重要意义。
Objective To investigate the changes of serum IL-27 in children with Mycoplasma pneumoniae pneumonia (MPP) and its significance in the pathogenesis of MPP. Methods Fifteen healthy children were included in this study. The levels of IFN-γ, IL-4 and IL-27 in peripheral blood were measured in 36 children with MPP and 35 children with non-mycoplasma pneumoniae pneumonia (NMPP). Serum levels of IFN-γ, IL-4 and IL-27 were measured by double antibody sandwich ELISA. Results The serum levels of IFN-γ in the acute phase of MPP group and NMPP group were (173.1 ± 29.2) pg / ml, (192.2 ± 36.4) pg / ml and (124.8 ± 34.7) pg / m, (100.4 ± 31.9) pg / ml and (80.0 ± 24.7) pg / ml respectively. The levels of IFN-γ and IL-4 in MPP group and NMPP group were higher than those in control group (P <0.05, P <0.01), but the ratio of IFN-γ / IL-4 in MPP group was decreased . The levels of IL-27 in MPP group, NMPP group and control group were (24.24 ± 9.00) pg / ml, (29.76 ± 10.18) pg / ml and <0.05), and NMPP group was higher than MPP group (P <0.05). The levels of IL-27 and IFN-γ in MPP group and NMPP group were positively correlated (r = 0.849, P <0.01). The serum IL-27 level was positively correlated with IFN-γ / IL- <0.01). Conclusions The acute phase of MPP in children with insufficient response to IL-27 and the imbalance of Th1 / Th2 response may play an important role in the immunopathological damage and extrapulmonary complication of MPP.