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目的研究糖皮质激素受体(glucocorticoid receptor,GR)亚型α和β在口腔扁平苔藓(oral lichen planus,OLP)中的表达特点,探讨GR与OLP发生及临床分型的关系。方法分别采用RT-PCR和免疫组化法检测GR亚型α和β在32例OLP患者(斑纹型18例,糜烂型14例)局部病损口腔黏膜和12例健康口腔黏膜的表达情况,并分析GRα/GRβ比值的差异。结果 GRα和GRβ均主要表达于OLP角质形成细胞。OLP病变口腔黏膜中GRαmRNA的表达水平降低(P=0.000),GRα/GRβ比值下降(P=0.000),而GRβmRNA的表达水平与健康对照组无统计学差异(P=0.07)。GRαmRNA、GRβmRNA及GRα/GRβ比值在斑纹型和糜烂型OLP中的表达均无统计学差异(分别为P=0.263,P=0.729,P=0.150)。结论 OLP中GR主要表达于局部病损区的角质形成细胞,GR亚型表达改变可能与OLP局部病损的发生有关。此外,GR亚型的表达水平可能与OLP病情的严重程度无关。
Objective To study the expression of glucocorticoid receptor α (α and β) in oral lichen planus (OLP) and to investigate the relationship between GR, OLP and clinical classification. Methods RT-PCR and immunohistochemistry were used to detect the expression of α and β of GR subtypes in the oral mucosa and 12 healthy oral mucosa of 32 patients with OLP (18 striae and 14 erosive lesions) Analysis of GRα / GRβ ratio differences. Results Both GRα and GRβ were mainly expressed in OLP keratinocytes. The expression of GRαmRNA in oral mucosa of OLP lesion decreased (P = 0.000) and the ratio of GRα / GRβ decreased (P = 0.000), while the expression of GRβmRNA showed no significant difference (P = 0.07) compared with healthy controls. There was no significant difference in the expression of GRαmRNA, GRβmRNA and GRα / GRβ between spiked and erosive OLP (P = 0.263, P = 0.729, P = 0.150). Conclusion GR in OLP is mainly expressed in keratinocytes in local lesion area. The change of GR subtypes may be related to the occurrence of local lesion in OLP. In addition, the expression of GR subtypes may not be related to the severity of OLP disease.