论文部分内容阅读
目的:以贯叶连翘为原料,应用响应面法探求超声提取金丝桃素的最优工艺。方法:在对影响金丝桃素提取率的单因素系统考察的基础上,采用部分析因设计分析筛选以上因素,并应用响应面法对其显著因素进行研究和优化。结果:建立了超声提取贯叶连翘中金丝桃素的工艺数学模型。超声提取金丝桃素的最佳工艺条件为超声提取时间36.15 min,丙酮/乙醇混合提取溶剂中丙酮体积分数为68.14%,液料比为11.87 mL.g-1,此时金丝桃素提取率理论值可达到1.454%。经验证,提取率为1.44%,与理论值相符。主效应关系为超声提取时间>丙酮体积分数>液料比。结论:通过以上试验得到的提取工艺条件可靠,金丝桃素提取率远高于常规提取方法,可为金丝桃素的工业化放大生产提供了一定的理论依据。
OBJECTIVE: To investigate the optimal extraction process of hypericin from Hypericum perforatum L. by using response surface methodology. Methods: Based on the investigation of the single factor system affecting the extraction rate of hypericin, the above factors were screened by partial factorial design and the significant factors were studied and optimized by response surface methodology. Results: The mathematical model of ultrasonic extraction of hypericin in Hypericum perforatum was established. The optimal extraction conditions for ultrasonic extraction of hypericin were as follows: ultrasonic extraction time 36.15 min, acetone-ethanol extraction solvent 68.14%, liquid-to-liquid ratio 11.87 mL.g-1, The theoretical value can reach 1.454%. Proven, the extraction rate was 1.44%, in line with the theoretical value. The main effect for ultrasonic extraction time> acetone volume fraction> liquid material ratio. Conclusion: The extraction process conditions obtained by the above test are reliable, and the extraction rate of hypericin is much higher than that of conventional extraction method, which can provide some theoretical basis for industrialized amplification of hypericin.