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目的:探讨新疆维吾尔族、汉族慢性牙周炎与冠状动脉粥样硬化性心脏病(以下简称冠心病)发病的相关性机制,比较两民族可能存在的差异。方法:收集36例行冠状动脉搭桥术并伴有慢性牙周炎的患者冠状动脉粥样硬化斑块和龈下菌斑,采用Chelex-100法提取细菌DNA,以聚合酶链反应(PCR)法检测冠状动脉粥样硬化斑块和龈下菌斑中牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)、伴放线放线杆菌(Actionbacillus actinomycetemcomitans,Aa)、具核梭杆菌(Fusobacterium nucleatum,Fn)、中间普氏菌(Preuotella intermedia,Pi)、福赛拟杆菌(Bacteroides forsythus,Bf)5种慢性牙周炎相关致病菌。结果:36例患者冠状动脉粥样硬化斑块中,各种牙周炎相关致病菌的检出率分别为:Pg36%,Aa3%,Pi14%,Fn25%,Bf47%;同一患者冠状动脉粥样硬化斑块及龈下菌斑中同时检出5种牙周炎相关致病菌的一致的例数:Bf15例,Pg13例,Fn15例,Pi3例,Aa0例;维吾尔族患者Bf、Pg的一致检出例数,明显高于汉族,差异具有统计学意义(p<0.05)。结论:在冠状动脉粥样硬化斑块中慢性牙周炎相关致病菌DNA的检出,提示慢性牙周炎相关致病菌在冠心病的发生、发展中可能起着重要的作用,两种慢性牙周感染致病菌(福赛拟杆菌、牙龈卟啉单胞菌)在动脉粥样硬化斑块中的检出率,维吾尔族高于汉族。
Objective: To investigate the correlation between chronic periodontitis and coronary atherosclerotic heart disease (hereinafter referred to as coronary heart disease) among Uygur and Han nationalities in Xinjiang and to compare the possible differences between the two ethnic groups. Methods: Thirty-six coronary atherosclerotic plaque and subgingival plaque in 36 patients undergoing coronary artery bypass grafting combined with chronic periodontitis were collected. Bacterial DNA was extracted by Chelex-100 method, and polymerase chain reaction (PCR) To detect Porphyromonas gingivalis (Pg), Actionbacillus actinomycetemcomitans (Aa), Fusobacterium nucleatum (Fn) in coronary atherosclerotic plaque and subgingival plaque, , Preuotella intermedia (Pi) and Bacteroides forsythus (Bf). Results: In 36 patients with coronary atherosclerotic plaque, the detection rate of various periodontitis-associated pathogens were: Pg36%, Aa3%, Pi14%, Fn25%, Bf47%; the same patients with coronary atherosclerosis Like plaques and subgingival plaque in the same time, there were 5 consistent cases of periodontitis-related pathogens: 15 cases of Bf, 13 cases of Pg, 15 cases of Fn, 3 cases of Pi and 0 cases of Aa. The Uygur patients with Bf and Pg The number of cases detected consistently was significantly higher than that of Han people, the difference was statistically significant (p <0.05). Conclusion: The detection of chronic periodontitis-associated DNA in coronary atherosclerotic plaques suggests that chronic periodontitis-associated pathogens may play an important role in the development of coronary heart disease. Two kinds of pathogens The detection rate of chronic periodontal pathogens (Bacteroides fastidiosa and Porphyromonas gingivalis) in atherosclerotic plaques was higher in Uyghur than in Han.