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近2年本文作者从西非AIDS患者中分离的HIV包膜糖蛋白的抗原性、核酸与中非、北美、欧洲分离的HIV不同,据此将后者称HIV-1,前者则为HIV-2。本文报道,30例西非患者的临床、免疫及病毒学研究资料。30例中17例为AIDS,研究时间为1985年9月～1986年9月,在此期间有7例死亡,男性12例,女性18例,平均年龄35岁,均已定居西非几年。在12例患者的淋巴细胞中分离HIV,以间接免疫荧光法(IFA)测定HIV-2抗体,用ELISA测定HIV-1抗体,以放射免疫沉淀测定法(RIPA)分析HIV-1及HIV-2抗原,用HIV-1探针(含LAV_(BRU)的全部基因组)及HIV-2探针(含LAV-2_(RUD)互补DNA的2Kb)进行核酸杂交。
In the recent two years, the authors isolated antigenicity of HIV envelope glycoprotein from AIDS patients in West Africa. Nucleic acid is different from HIV isolated in Central Africa, North America and Europe. According to this, the latter is called HIV-1, while the former is HIV-2 . This article reports 30 cases of West Africa patients clinical, immunological and virological data. Seventeen of 30 patients were AIDS and the study period was from September 1985 to September 1986. During this period, seven patients died, 12 males and 18 females, with an average age of 35 years and had settled in West Africa for several years. HIV was isolated from lymphocytes of 12 patients, HIV-2 antibody was detected by indirect immunofluorescence (IFA), HIV-1 antibody was measured by ELISA, and HIV-1 and HIV-2 were analyzed by radioimmunoprecipitation (RIPA) Antigens were hybridized with HIV-1 probe (whole genome containing LAV BRU) and HIV-2 probe (2 Kb containing LAV-2 RUD complementary DNA).