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目的了解肠出血性大肠埃希菌O157︰H7在东台市动物宿主和腹泻患者中带菌及毒力基因携带与耐药情况。方法于流行季节采集动物宿主、腹泻患者粪便及生熟食品和苍蝇标本,mEC肉汤增菌后,用特异性免疫磁珠集菌、科玛嘉显色平板分离,纯化的菌株经生化鉴定,血清分型,以K-B纸片法进行药敏试验,采用PCR方法检测uida基因和stx1、stx2、eaeA、hly等4种毒力基因。结果 683份标本共检出O157︰H7菌株35株,检出率为5.12%,其中动物粪便检出率为10.59%(34/321),检出率最高的为牛粪(26.92%),其次为山羊粪(7.50%)和猪粪(7.32%),腹泻患者的检出率为0.39%(1/256)。35株菌株均检出eaeA和hly毒力基因,而牛粪中stx2、eaeA和hly等3种毒力基因组合型检出率达12.82%。35株菌株对氨苄西林敏感率为14.3%,对其余17种抗生素敏感率均>90%。结论东台市动物宿主和腹泻患者均不同程度携带肠出血性大肠埃希菌O157︰H7,且存在流行的潜在危险。
Objective To understand the carrier and drug resistance of enterohemorrhagic Escherichia coli O157︰H7 in host and diarrhea patients in Dongtai. Methods In the epidemic seasons, animal feces, raw and cooked foods and flies were collected from diarrhea patients. After enrichment with mEC broth, the bacteria were collected with special immunomagnetic beads and purified by Kema Jia chromogenic plate. The purified strains were biochemically identified, Serotyping, drug sensitivity test using KB paper method, using the PCR method to detect uida gene and stx1, stx2, eaeA, hly and other four virulence genes. Results A total of 35 strains of O157: H7 were detected in 683 samples, the detection rate was 5.12%. The detection rate of animal feces was 10.59% (34/321), the highest detection rate was cow dung (26.92%), followed by For goat manure (7.50%) and pig manure (7.32%), the detection rate of diarrhea patients was 0.39% (1/256). 35 strains were detected eaeA and hly virulence genes, and cow dung stx2, eaeA and hly and other three virulence gene combination detection rate of 12.82%. 35 strains of ampicillin sensitive rate of 14.3%, the other 17 kinds of antibiotics susceptibility> 90%. Conclusion The host animal and diarrhea patients in Dongtai city all carry enterohemorrhagic Escherichia coli O157︰H7 to varying degrees, and there is a potential risk of epidemic.