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目的:研究胆囊癌及炎性组织细胞膜上生物大分子蛋白质及脂质的红外光谱改变。方法:应用傅里叶变换红外光谱技术(Fourier transformed infare spectroscopy,FTIR)对胆囊癌和炎性组织的细胞膜进行检测,并分析其蛋白质和脂质的红外变化。结果:胆囊癌和炎性组织细胞膜上生物大分子蛋白质和脂质的红外光谱存在明显差异:癌组织中酰胺Ⅱ带较弱、峰型低平,酰胺Ⅰ带较强、峰型宽大,相对吸收强度I1650/I1550明显升高;癌组织中与脂质相关的吸收谱带出现概率高、且峰型高尖,相对吸收强度I2957/I1650和I1740/I1400的比值增加;相对吸收强度I1460/I1400的比值在正常组织细胞膜中较高。结论:胆囊炎性与癌组织细胞膜上大分子红外光谱存在不同,通过FTIR能对细胞癌变早期生物大分子改变做出客观反映。
Objective: To study the infra-red spectrum changes of biomacromolecules and lipids in gallbladder carcinoma and inflammatory cell membrane. Methods: The cell membrane of gallbladder carcinoma and inflammatory tissues were detected by Fourier transform infraction spectroscopy (FTIR), and the infrared and infrared spectra of protein and lipid were analyzed. Results: There were significant differences in the infrared spectra of the macromolecule proteins and lipids in the gallbladder carcinoma and the inflammatory cell membrane. The amide Ⅱ band in cancer tissues was weak, the peak shape was low, the amide Ⅰ band was strong, the peak shape was broad and the relative absorption The intensity of I1650 / I1550 increased significantly; the probability of occurrence of lipid-related absorption bands in cancerous tissues was high and the peak shape was sharp; the ratio of relative absorption intensity, I2957 / I1650 and I1740 / I1400, increased; the relative absorption intensity, I1460 / The ratio is higher in normal cell membranes. Conclusion: There is a difference between the cholecystitis and the macromolecular infra-red spectrum in the cell membrane of cancer. The FTIR can objectively reflect the changes of early biological macromolecules in cancerous cells.