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观察环孢素A防止血管内膜增生作用与单核细胞趋化蛋白-1(Monocyte chemoattractant protein-1,MCP-1)水平的关系。雄性SD大鼠36只,随机分为假手术组(n=12)、手术组(n=12)、干预组(n=12)。术后30 d取实验材料,血管组织作HE染色;荧光定量PCR技术检测损伤血管壁钙调神经磷酸酶(Calcineurin,Ca N)、活化T细胞核因子3(Nuclear factor of activated T cell,NFATc3)、MCP-1在mRNA水平上的表达变化;ELISA法测定血浆MCP-1在蛋白水平上的表达变化。假手术组内膜无增生,手术组血管壁内膜明显增生,干预组内膜亦出现增生,但内膜增生、内膜/中膜厚度与手术组相比较明显减轻(P<0.05)。干预组与手术组相比较,血管壁组织Ca N、NFATc3、MCP-1 mRNA表达明显减少(P<0.05)。血浆MCP-1水平干预组与手术组相比明显降低(P<0.05)。环孢素A通过抑制Ca N-NFATc信号通路减轻受损血管壁以及血浆的炎症因子MCP-1水平,从而减轻球囊损伤术后动脉内膜增生。
To investigate the relationship between cyclosporin A and neointimal hyperplasia and monocyte chemoattractant protein-1 (MCP-1) levels. Thirty - six male SD rats were randomly divided into sham operation group (n = 12), operation group (n = 12) and intervention group (n = 12). At 30 days after operation, the experimental materials were taken for HE staining. Fluorescent quantitative PCR was used to detect the expression of calcineurin (Ca N), activated nuclear factor of activated T cell (NFATc3) The expression of MCP-1 at the mRNA level was measured. The protein expression of MCP-1 in plasma was detected by ELISA. There was no hyperplasia in the intima of the sham operation group, hyperplasia of the intima in the operation group and hyperplasia of the intima in the intervention group. However, intima hyperplasia and intima / media thickness were significantly reduced compared with the operation group (P <0.05). Compared with the operation group, the expressions of Ca N, NFATc3 and MCP-1 mRNA in the vascular wall were significantly decreased (P <0.05). Plasma MCP-1 levels in intervention group were significantly lower than those in operation group (P <0.05). Cyclosporine A attenuated the intimal hyperplasia of the artery after balloon injury by inhibiting the CaN-NFATc signaling pathway and attenuating the MCP-1 level in the damaged vascular wall and plasma.