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目的建立一种同时测定蜜饯中7种常见合成色素(柠檬黄、苋菜红、胭脂红、日落黄、亮蓝、诱惑红、赤藓红)的固相萃取-高效液相色谱(solid-phase extraction-high performance liquid chromatography,SPE-HPLC)分析方法。方法样品经水提取后,采用Anpelclean PA聚酰胺SPE小柱净化,氮吹后定容,以甲醇和20mmol/L乙酸铵为流动相,通过Waters公司XBridge C18柱梯度洗脱分离,在特异性波长下进行测定。结果 7种常见合成色素在0.50~10.0μg/m L范围内峰面积与其实际浓度线性相关性良好(r2≥0.9987),在0.5、2.5、5.0mg/kg三个加标水平上回收率在74.3%~97.5%之间,RSD在0.47%~4.80%之间。检出限在0.02~0.10 mg/kg之间,定量限在0.06~0.30 mg/kg之间。结论本方法灵敏度高,分离效果好,精密度好,准确度高,适用于蜜饯中7种常见合成色素的测定。
OBJECTIVE To establish a solid-phase extraction (RP-HPLC) method for the simultaneous determination of seven common synthetic pigments (lemon yellow, amaranth, carmine, sunset yellow, brilliant blue, -high performance liquid chromatography, SPE-HPLC). Methods The samples were extracted with water and purified by Anpelclean PA polyamide SPE cartridge. After the nitrogen was purged, methanol and 20mmol / L ammonium acetate were used as the mobile phase and separated by gradient elution on a Waters XBridge C18 column. The specific wavelength Under the determination. Results The peak area of 7 common synthetic pigments in the range of 0.50 ~ 10.0 μg / m L was linearly correlated with their actual concentration (r2 ≥ 0.9987). The recoveries at the three spiked levels of 0.5, 2.5 and 5.0 mg / kg were 74.3 % ~ 97.5%, RSD between 0.47% ~ 4.80%. The limit of detection was between 0.02 and 0.10 mg / kg, and the limit of quantitation was between 0.06 and 0.30 mg / kg. Conclusion The method has high sensitivity, good separation effect, good precision and high accuracy. It is suitable for the determination of seven common synthetic pigments in candied fruit.