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大鼠心肌缺血 /再灌注损伤是模拟人类心梗及溶栓再通治疗 ,研究缺血预适应机制 ,评价抗心律失常药物疗效常用的动物模型。本文在传统造模方法的基础上进行了一定的改进 ,采用在压管下加一小垫片的方法 ,减少了传统推管法对心表面的机械损伤 ,方法简便 ,冠脉阻断确实。结果 :大鼠左冠状动脉主干缺血30′,再灌 12 0′,危险区 /左室重量比为 5 5 %± 5 % ,坏死区 /危险区为 5 8%± 6 % (N =10 )。心肌酶谱结果 :CK430 1± 2 5 1u/l,CK- MB 2 2 88± 32 8u/l,L DH 2 32 9± 2 16 u/l,AST 371± 5 7u/l(N=5 )。
Myocardial ischemia / reperfusion injury in rats is a kind of animal model that simulates human myocardial infarction and recanalization by thrombolysis, studies ischemic preconditioning mechanism and evaluates antiarrhythmic drug efficacy. In this paper, some improvements have been made on the basis of the traditional method of modeling. The method of adding a small pad under the tube reduces the mechanical damage to the heart surface by the traditional push tube method, and the method is simple and the coronary artery occlusion is sure. Results: The left main coronary artery of the rats were ischemia 30 and reperfusion 120, the ratio of risk area to left ventricle was 5 5% ± 5%, necrosis area / risk area was 58% ± 6% (N 10 ). Myocardial zymogram results: CK430 1 ± 25 1u / l, CK-MB 2 2 88 ± 32 8u / l, L DH 2 32 9 ± 2 16 u / l, AST 371 ± 5 7u / l (N = 5) .