为了研究凤梨科植物开花的机理,本研究利用5’RACE和3’RACE方法从蜻蜓凤梨中克隆得到响应开花的主要调控基因FT1。该基因CDS序列全长为625 bp。为了进一步研究其功能,本研究将其重组并构建了以CaMV35S为启动子、GUS为报告基因的植物表达载体CaMV35S-GUS-FT1,并采用农杆菌介导的方法转化云烟K346烟草的幼嫩叶片,经GUS组织化学染色观察转基因烟草体细胞中GUS报告基因的表达情况。研究结果验证了重组构建的FT1基因载体的正确性,为进一步解析FT1基因在蜻蜓凤梨开花过程中的功能奠定了基础。 In order to study the mechanism of the flowering of the bromeliads, we used the 5’RACE and 3’RACE methods to clone the major regulatory gene FT1 from the dragonfly pineapple. The CDS sequence of this gene is 625 bp in length. In order to further study its function, this study recombined and constructed a plant expression vector CaMV35S-GUS-FT1 with CaMV35S as promoter and GUS as reporter gene and Agrobacterium-mediated transformation of young leaves of Yunfu K346 tobacco GUS histochemical staining was used to observe the expression of GUS reporter gene in transgenic tobacco somatic cells. The results verify the correctness of the constructed FT1 gene vector and lay a foundation for further analysis of the function of FT1 gene in the flowering process of the dragonfly pineapple.