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目的研究血管内皮生长因子(VEGF)与基质金属蛋白酶-7(MMP-7)在直肠癌中的表达、相关性及与直肠癌恶性度、转移、分期的关系。方法采用RT-PCR半定量技术,对30例直肠癌的手术切除标本(包括癌组织及其相应正常黏膜)中的VEGF及MMP-7基因表达进行检测。结果VEGF在26例直肠癌组织和15例正常黏膜中表达;29例直肠癌组织中MMP-7mRNA表达阳性,1例正常黏膜中表达。直肠癌的不同分期与VEGF和MMP-7表达有关,随癌分期进展而呈现阳性结果增高(P<0.05)。结论VEGF及MMP-7在直肠癌组织中均有差异性表达,是癌发生、生长和转移的重要因素,在直肠癌的生物学研究中可作为判别指标。
Objective To investigate the expression and correlation of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-7 (MMP-7) in rectal cancer and its relationship with the malignancy, metastasis and staging of rectal cancer. Methods RT-PCR was used to detect the expression of VEGF and MMP-7 in 30 resected specimens of rectal cancer, including cancer tissue and its corresponding normal mucosa. Results The expression of VEGF was detected in 26 cases of rectal cancer and 15 cases of normal mucosa. The expression of MMP-7 mRNA in 29 cases of rectal cancer was positive and in 1 case of normal mucosa. The different stages of rectal cancer were related to the expression of VEGF and MMP-7, and the positive results were higher with the progress of cancer staging (P <0.05). Conclusions VEGF and MMP-7 are differentially expressed in rectal cancer tissues and are important factors of carcinogenesis, growth and metastasis. They can be used as a discriminant in the biological study of rectal cancer.