在最近完成测序的水稻籼稻和粳稻两个亚种基因组中 ,各找到 5 6 4和 5 19个较为可靠的tRNA基因 ,进一步证实了于 2 0 0 2年发表的基于基因组序列草图的分析结果。修正的摆动假设 ,即至少需要 4 6种tRNA基因才能译出 6 1种可能的反密码子 ,在这两个亚种中均准确成立。在这 4 6种tRNA中 ,有些在籼稻和粳稻中的序列均全同。有 18种水稻tRNA与拟南芥中的相应序列全同。在籼稻基因组序列中还发现了 384个 5SrRNA基因 ,一批 17S和5 .8SrRNA基因以及一个 2 5SrRNA基因。这些rRNA基因的不完备是由于它们通常以串接阵列形式存在于异染色质区域 ,而后者在全基因组霰弹法测序中不易完整测出。在tRNA和rRNA基因序列之间发现了多处互补片段 ,这将有助于研究它们的进化和相互作用。 A total of 544 and 519 more reliable tRNA genes were found in the genomes of the two subspecies of indica and japonica rice recently sequenced, further confirming the results of genome-based sequence sketches published in 2002. The modified swing hypothesis that at least 46 tRNA genes are required to translate 61 possible anticodons is well established in both subspecies. Of the 46 tRNAs, some are identical in both indica and japonica. Eighteen rice tRNAs are identical to the corresponding sequences in Arabidopsis. 384 5S rRNA genes, a group of 17S and 58SrRNA genes and a 25SrRNA gene were also found in the indica genome sequence. The incompleteness of these rRNA genes is due to the fact that they are usually found in heterochromatin regions in tandem arrays that are not easily detected intact in genome-wide shotgun sequencing. Multiple complementary fragments were found between the tRNA and rRNA gene sequences, which will help to study their evolution and interaction.