目的:研究黔产鱼眼草的化学成分,为阐明其活性成分提供依据。方法:利用各种色谱技术进行分离,根据化合物的光谱数据和理化常数鉴定其结构。结果:分离并鉴定了7个化合物,分别为正二十二烷(1)、正二十八烷酸(2)、正二十烷酸甲酯(3)、十六烷酸三十烷醇酯(4)、脱镁叶绿素甲酯(5)、柳穿鱼素(6)、柳穿鱼素-7-葡萄糖苷(7)。结论:化合物1～7均为首次从该植物中分离得到。 OBJECTIVE: To study the chemical composition of Eucalyptus grandis and provide basis for clarifying its active ingredients. Methods: Separation was performed using various chromatographic techniques, and the structures were identified based on the spectral data and physiochemical constants of the compounds. RESULTS: Seven compounds were isolated and identified, n-docosane (1), n-octacosanoic acid (2), methyl n-eicosanoate (3), and triacontanyl hexadecyl alcohol. Ester (4), pheophorbide methyl ester (5), thiourethrin (6), and thionidin-7-glucoside (7). Conclusion: Compounds 1-7 were isolated from this plant for the first time.