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目的研究通过手工计数精子浓度质控不同实验室计算机辅助精液分析(CASA)检测精子浓度,以保证不同实验室之间检测的精子浓度标准的统一性。方法 2016年1月1日至2016年3月31日,通过手工计数和CASA两种方法计数3家医院共600份精液的精子浓度,通过分析比较手工计数与CASA检测结果的相关性。根据手工计数的结果,对于CASA的相关参数进行相应校正。2016年4月1日至2016年6月30日,再次通过手工计数和CASA两种方法计数3家医院共600份精液的精子浓度,分析比较手工计数与CASA检测的结果。结果 (1)2016年1月1日至2016年3月31日,检测的600份精液标本,其中同济大学附属第一妇婴保健院400份,手工计数和CASA两种方法检测精子浓度,差异没有统计学意义(P>0.05);上海市浦东新区妇幼保健院100份,手工计数的结果高于CASA检测的结果,差异具有统计学意义(P<0.05);上海市闵行区妇幼保健院100份,手工计数的结果低于CASA检测的结果,差异具有统计学意义(P<0.05)。(2)根据以上检测结果,对于CASA的相关参数进行相应校正。2016年4月1日至2016年6月30日,检测的600份精液标本,其中同济大学附属第一妇婴保健院400份,手工计数和CASA两种方法检测精子浓度,差异没有统计学意义(P>0.05);上海市浦东新区妇幼保健院100份,差异没有统计学意义(P>0.05);上海市闵行区妇幼保健院100份,差异没有统计学意义(P>0.05)。结论通过对于不同实验室手工计数精子浓度的标准统一化,来定期校正CASA的检测结果,以达到保证不同实验室之间CASA检测精子浓度的准确性和一致性。
Objective To measure the concentration of sperm in different laboratories by computer-assisted semen analysis (CASA) by hand counting the concentration of sperm in order to ensure the consistency of sperm concentration standards among different laboratories. Methods From January 1, 2016 to March 31, 2016, the sperm concentration of 600 sperm samples from 3 hospitals was counted by manual counting and CASA. The correlation between manual counting and CASA was analyzed and compared. According to the result of manual counting, the related parameters of CASA should be corrected accordingly. From April 1, 2016 to June 30, 2016, the sperm concentration of 600 sperm samples from 3 hospitals was counted again by manual counting and CASA. The results of manual counting and CASA were analyzed and compared. Results (1) From January 1, 2016 to March 31, 2016, 600 semen samples were detected, of which 400 were from the First Affiliated Maternity and Infancy Hospital of Tongji University. The manual counting and CASA were used to detect the sperm concentration. The differences There was no statistical significance (P> 0.05). The results of manual counting in 100 samples of MCH in Shanghai Pudong New Area were higher than those of CASA (P <0.05). The MCH 100 in Shanghai Minhang District The results of manual counting were lower than the results of CASA test, the difference was statistically significant (P <0.05). (2) According to the above test results, the relevant parameters for CASA corresponding correction. From April 1, 2016 to June 30, 2016, 600 semen samples were detected, including 400 maternal and child health-care centers attached to Tongji University. There was no significant difference between the two methods in hand counting and CASA (P> 0.05). There was no significant difference in 100 maternity and child care hospitals in Pudong New Area in Shanghai (P> 0.05). There was no significant difference in 100 maternity and child care hospitals in Minhang district, Shanghai (P> 0.05). Conclusions The CASA test results were regularly calibrated by standardizing the standardization of hand-counted sperm concentrations in different laboratories to ensure the accuracy and consistency of CASA-detected sperm concentrations among different laboratories.