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[目的]研究microRNA-133a(miR-133a)在5-氟尿嘧啶(5-Fu)诱导胃癌细胞BGC823及SGC7901凋亡中的作用。[方法]用荧光定量PCR检测经5-Fu(IC50值)处理过胃癌细胞miR-133a的表达;构建过表达miR-133a的胃癌细胞株并行细胞增殖、细胞流式验证miR-133a的生物学功能;5-Fu分别处理过表达及敲低miR-133a的胃癌细胞株后,行细胞增殖、细胞流式、Western Blot观察细胞生长及凋亡情况。[结果]5-Fu诱导BGC823及SGC7901内源性miR-133a的表达(>50%,P<0.0001,24h),miR-133a促进胃癌细胞凋亡,敲低miR-133a可以抑制5-Fu对胃癌细胞的促凋亡作用。[结论]5-Fu通过诱导胃癌细胞BGC823及SGC7901内源性miR-133a的表达,促进胃癌细胞凋亡。
[Objective] To investigate the role of microRNA-133a (miR-133a) in the apoptosis of BGC823 and SGC7901 gastric cancer cells induced by 5-fluorouracil (5-Fu). [Methods] The expression of miR-133a in gastric cancer cells treated with 5-Fu (IC50 value) was detected by real-time quantitative PCR. The parallel proliferation and proliferation of gastric cancer cell lines overexpressing miR- 5-Fu was used to treat gastric cancer cell lines overexpressing and knocking down miR-133a respectively. Cell proliferation and flow cytometry were used to observe the cell growth and apoptosis. [Results] 5-Fu induced the expression of endogenous miR-133a (> 50%, P <0.0001, 24 h) in BGC823 and SGC7901 cells. MiR-133a promoted the apoptosis of gastric cancer cells. Apoptosis of gastric cancer cells. [Conclusion] 5-Fu can promote gastric cancer cell apoptosis by inducing endogenous miR-133a expression in BGC823 and SGC7901 gastric cancer cells.