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荧光素汞在碱性条件下具有很强的荧光,H2S能与荧光素汞结合,使其荧光猝灭,据此建立了一种荧光法测定大鼠肠灌流液中H2S含量的方法。在0.1mol·L-1 NaOH溶液中,以Na2S作为H2S供体,荧光素汞浓度为5.0×10-5 mol·L-1,Na2S浓度为1.0×10-5 mol·L-1时,以498nm为激发波长,在522nm处测定此二元体系的荧光强度。结果表明,在4.0×10-7~2.0×10-6 mol·L-1范围内,H2S浓度与荧光强度的下降程度呈良好的负相关性,r=0.998 0,精密度实验RSD=4.59%(n=7),检出限3.5×10-8 mol·L-1,样品中H2S含量分别为1.01×10-6和1.15×10-6 mol·L-1,加标回收率为95.8%~101.0%。该方法操作简单,灵敏度高,稳定性好,可准确测定肠灌流液中H2S含量,为内源性H2S的测定提供了依据。
Fluorescein mercury has a strong fluorescence under alkaline conditions, and H2S can combine with fluorescein-mercury to quench its fluorescence. Thus, a fluorescence method for the determination of H2S in intestinal perfusate was established. In 0.1 mol·L-1 NaOH solution, Na2S was used as the donor of H2S, the concentration of mercury was 5.0 × 10-5 mol·L-1 and the concentration of Na2S was 1.0 × 10-5 mol·L-1 498nm for the excitation wavelength, measured at 522nm fluorescence intensity of the binary system. The results showed that there was a good negative correlation between the concentration of H2S and the decrease of fluorescence intensity in the range of 4.0 × 10-7 ~ 2.0 × 10-6 mol·L-1, r = 0.998 0, precision test RSD = 4.59% (n = 7). The detection limit was 3.5 × 10-8 mol·L-1. The content of H2S in the sample was 1.01 × 10-6 and 1.15 × 10-6 mol·L-1, respectively, and the recoveries were 95.8% ~ 101.0%. The method has simple operation, high sensitivity and good stability, and can accurately determine the content of H2S in intestinal perfusate, which provides the basis for the determination of endogenous H2S.