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目的:通过检测分析血清及脑脊液中肿瘤坏死因子-ɑ(TNF-ɑ)及其受体在病毒性脑炎患儿中治疗前后的浓度变化,探寻TNF-ɑ在病毒性脑炎发病中的作用机制。方法:对象为本院患者中随机选取的15例重症与10例轻症患病毒性脑炎患儿和10例股疝患儿。用ELISA方法在治疗前测定血清和脑脊液以及在治疗后血清当中TNF-ɑ的浓度并进行比较分析。结果:患儿血清和脑脊液中TNF-ɑ浓度在轻症组和重症组中和治疗前相比明显升高,升高程度重症组大于轻症组,轻症组大于对照组(p<0.01),差异有统计学意义。治疗后,重症组和轻症组患儿中血清TNF-ɑ浓度与治疗前相比明显下降(p<0.01),统计学有显著差异。结论:病毒性脑炎患儿血清和脑脊液中TNF-ɑ水平与疾病严重程度呈正相关,治疗前浓度越高病情越严重。
OBJECTIVE: To explore the role of TNF-ɑ in the pathogenesis of viral encephalitis by detecting the changes of tumor necrosis factor-ɑ (TNF-ɑ) and its receptor in serum and cerebrospinal fluid before and after treatment in children with viral encephalitis mechanism. Methods: The subjects were 15 randomly selected from our hospital and 10 children with mild viral encephalitis and 10 children with hernia. Serum and cerebrospinal fluid were measured by ELISA before treatment and TNF-ɑ concentrations in the serum after treatment were compared and analyzed. Results: Serum and cerebrospinal fluid concentrations of TNF-患 in children with mild and severe disease were significantly higher than those before treatment, and those with severe disease were higher in the severe group than in the mild group and those in the mild group (p <0.01) ,The difference was statistically significant. After treatment, the levels of serum TNF-ɑ in severe group and mild group were significantly lower than those before treatment (p <0.01), and there was a statistically significant difference. Conclusion: The level of TNF-血 in serum and cerebrospinal fluid of children with viral encephalitis is positively correlated with the severity of the disease. The higher the concentration before treatment, the more severe the disease.