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Tumor-initiating cells(TICs)are a highly tumorigenic subpopulation of solid tumor cells that play a critical role in the initiation of cancer~[1].These tumorigenic cells resist conventional chemotherapeutic drug treatment and are assumed to be playing major roles in cancer relapses after chemotherapy~[2].However,the notion of TICs has been rather controversial.A report shows that a high percentage(>25%)of human melanoma cells can generate a tumor in a NOD-SCID interleukin-2 receptor gamma chain null mouse~[3],suggesting that there is no clonal development of solid tumors,refuting the idea of TICs.We recently developed a method of isolating TICs from cancer cell lines by culturing single individual cells of B16-F1(a melanoma cell line)into 3D soft fibrin gels~[4].In addition to being able to generate local tumors in a wild-type
Tumor-initiating cells (TICs) are a highly tumorigenic subpopulation of solid tumor cells that play a critical role in the initiation of cancer ~ [1]. These tumorigenic cells resist conventional chemotherapeutic drug treatment and are assumed to be playing major roles in cancer relapses After chemotherapy ~ [2]. However, the notion of TICs has been quite controversial. A report shows that a high percentage (> 25%) of human melanoma cells can generate a tumor in a NOD-SCID interleukin-2 receptor gamma chain null suggesting that there is no clonal development of solid tumors, refuting the idea of TICs. We recently developed a method of isolating TICs from cancer cell lines by culturing single individual cells of B16-F1 (a melanoma cell line) into 3D soft fibrin gels ~ [4]. Addition to being able to generate local tumors in a wild-type