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目的采用HPLC-PDA技术和“化学模糊识别”策略对丹参-红花药对不同制法、不同配比的活性物质变化规律进行了系统研究。方法通过建立丹参与红花的化学物质库,并经过对照品峰的紫外信息,建立了4类活性物质的紫外信号网络,并通过网络将丹参-红花中的成分进行快速鉴别与归类,进而通过对称配比设计对这些化合物进行定量分析。结果在丹参-红花配伍的水、50%甲醇水和甲醇煎液中,通过“化学模糊识别”策略分别鉴定出47、57和43个成分。3种不同制法中,丹参酮类成分在甲醇提取液中的相对溶出度最高,其他3类成分在50%甲醇水提取液中的相对溶出度最高。相同制法、不同配比中,所测成分总的相对溶出度的优化范围皆为5∶1~5∶2,总的相对溶出度的最高比例皆为5∶2。结论丹参-红花不同配比与不同制法的化学成分的配伍变化规律有所不同,且存在优化配比,为丹参-红花药对临床配伍合理应用提供了参考,为药对现代基础研究提供了思路与方法。
OBJECTIVE: To systematically study the changes of the active substances of Salvia miltiorrhiza-safflower on different preparation methods and different proportions by using HPLC-PDA technology and “chemical fuzzy recognition” strategy. Methods By establishing the chemical substance database of Salvia miltiorrhiza and safflower, and through the ultraviolet information of reference substance peak, the UV signal network of four kinds of active substances was established and the components in Salvia miltiorrhiza - safflower were rapidly identified and classified by the network. These compounds were then quantified by symmetric ratio design. Results 47, 57 and 43 components were identified by the “chemical fuzzy recognition” strategy in the water of Salvia miltiorrhiza - safflower compatibility, 50% methanol water and methanol. Among the three different preparation methods, the relative dissolution rate of tanshinones in methanol extract was the highest, while the relative dissolution rate of the other three components in 50% methanol water extract was the highest. In the same preparation method and different mixture ratio, the optimum relative dissolution of the tested components is in the range of 5: 1 ~ 5: 2, and the highest relative total dissolution rate is 5: 2. Conclusion The compatibility of different compositions of Salvia miltiorrhiza and safflower is different from that of the different preparation methods, and there is an optimal ratio, which provides a reference for the rational application of Salvia miltiorrhiza - safflower in clinical compatibility. Provide ideas and methods.