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目的 获得中国流行株GⅡ-4型诺如病毒(NoV)ORF2基因序列,为进一步研究诊断、疫苗提供基础依据.方法 从GⅡ-4型NoV粪便中提取病毒RNA,经逆转录后PCR并克隆到simple PMD18-T载体获得完整开放读码柜架(ORF2)阅读框序列.结果 获得了正确序列的基因Ⅱ-4,亚型(genogroup Ⅱ-4,GⅡ-4)NOV ORF2基因序列,经软件分析,3株NOV ORF2序列编码的氨基酸大小约为54KD,与VA387(GⅡ-4)氨基酸同源性为92%～93%.ORF2编码的氨基酸序列存在3个相对保守序列.结论 获得GⅡ-4型NoV ORF2基因序列,为进一步表达病毒衣壳蛋白及Nov感染的诊断试剂及防治疫苗药物研制提供基础依据.“,”Objective To obtain the open reading frame 2 (ORF2) sequence of norovims GⅡ-4 strain for further study on diagnosis and vaccine of norovirus.Methods RNA was extracted from fecal sample.After transcription and PCR, the ORF2 gene was cloned into simple T-vector.The sequences were analyzed with DNA Star software and compared with pro-totype sequences retrieved from the NCBI database by Clustal X and Treeview software.Results The exact whole ORF2 gene of norovims GⅡ-4 swain was obtained which coding 54 KD amino acid.Three strains in this study showed 92% -93% homology with VA387 strains and 3 conserved regions.Conclusion ORF2 gene of norovirus GⅡ-4 strain was obtained, which will lay the basis for further study on diagnosis and vaccine of norovirus.