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目的:探索分枝杆菌噬菌体Guo1对结核休眠菌的复苏作用.方法:采用缺钾Sauton培养基培养对数生长期结核菌构建结核休眠菌模型;采用药敏检测、透射电子显微镜(电镜)观察和金胺O-尼罗红双荧光染色作为模型检测方法;采用菌落计数作为复苏的检测指标.结果:缺钾结核休眠菌模型构建成功.混合培养第1天时,空白对照组、上清组、TM4组、Guo1组菌落计数分别为(2.67±0.58)× 102、(3.00± 1.00)×102、(2.67±0.58)× 102、(2.33±0.58)×102 CFU/mL;培养第15天时,各组菌落计数分别为(7.33±1.53)×104、(6.67±2.08)×1010、(3.00± 1.00)× 1010、(3.33±1.53)× 1010CFU/mL.结论:分枝杆菌噬菌体Guo1能够复苏结核休眠菌.“,”Objective:To investigate the resuscitative potential of mycobacteriophage Guo1 on dormant cells of M.tuberculosis.Methods:Dormant cells of M.tuberculosis were induced by potassium-deficient culture of logarithmic phase of M.tuberculosis at 37 ℃,which were proven through drug sensitivity test,transmission electron microscope(TEM) and auramine O-Nile red double fluorescent staining.M.tuberculosis colony count was used to detect whether the dormant cells of M.tuberculosis had been resuscitated by Guo1.Results:Dormant cells of M.tuberculosis were obtained successfully by potassium-deficient cultivation for 30 days at 37 ℃.The concentration of M.tuberculosis in control,supernatant (SN),TM4 and Guo1 groups was (2.67±0.58)×102,(3.00±1.00)×102,(2.67±0.58)×102,(2.33±0.58)×102 CFU/mL,respectively,after culture for 1 d.And the concentration of M.tuberculosis in above groups increased to (7.33±1.53)×104,(6.67±2.08)×1010,(3.00±1.00)×1010,(3.33±1.53)×1010 CFU/mL at the 15th day,respectively.Conclusion:Mycobacteriophage Guo1 can resuscitate dormant cells of M.tuberculosis.