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目的:观察甲基化转移酶抑制剂5-氮杂-2’-脱氧胞苷(5-aza-2dc)联合多西紫杉醇(DT)对人前列腺癌(PCa)细胞系PC3细胞增殖、迁移、侵袭能力、细胞周期变化和细胞凋亡的影响,探讨上述两种药物联合应用对前列腺癌细胞的体外抗肿瘤效应。方法:实验分为对照组、5-aza-2dc组、DT组及5-aza-2dc和DT联合用药组,采用四甲基偶氮唑蓝(MTT)比色法、划痕损伤实验、细胞迁移实验分别观察5-aza-2dc和(或)DT对PC3细胞株增殖、迁移、侵袭能力的抑制效应,利用Annexin V-FITC/PI染色和流式细胞术检测其诱导肿瘤细胞凋亡的作用及其与细胞周期的关系。结果:5-aza-2dc组、DT组及联合用药组均能明显提高对PC3细胞的抑制率,降低细胞迁移距离,减少侵入下室的细胞(P<0.05),联合用药组作用更明显;对照组、5-aza-2dc组、DT组及联合用药组细胞凋亡率分别为(10.65±0.39)%、(16.60±0.67)%、(17.95±1.08)%、(22.98±1.18)%,各用药组均能明显提高细胞凋亡率,联合用药组作用更明显(P<0.05);联合用药组能明显减少G0/G1期细胞,增加G2/M期细胞(P<0.05)。结论:5-aza-2dc和DT单独及联合应用均能在体外细胞水平显著抑制PC3细胞的增殖、迁移和侵袭作用,诱导细胞凋亡,使细胞停滞于G2/M期,两药联合的作用比各单药作用大,5-aza-2dc和DT联合作用于PC3细胞具有协同作用,值得进一步研究。
AIM: To investigate the effects of 5-aza-2dc combined with docetaxel on the proliferation and migration of human prostate cancer cell line PC3, Invasion ability, cell cycle changes and apoptosis, to explore the combination of these two drugs on prostate cancer cells in vitro anti-tumor effect. Methods: The experiment was divided into control group, 5-aza-2dc group, DT group and 5-aza-2dc combined with DT group. MTT assay, scratch injury test, Migration assay was used to observe the inhibitory effect of 5-aza-2dc and / or DT on the proliferation, migration and invasion ability of PC3 cells respectively. The apoptosis of tumor cells was detected by Annexin V-FITC / PI staining and flow cytometry And its relationship with the cell cycle. Results: The 5-aza-2dc group, DT group and combined treatment group could significantly increase the inhibition rate of PC3 cells, reduce the migration distance and reduce the invasion of cells (P <0.05) The apoptotic rates in control group, 5-aza-2dc group, DT group and combination group were (10.65 ± 0.39)%, (16.60 ± 0.67)%, (17.95 ± 1.08)% and (22.98 ± 1.18)%, The drug combination group could obviously increase the apoptosis rate, and the effect of combination group was more obvious (P <0.05). The combined treatment group could obviously reduce the number of G0 / G1 phase cells and increase the G2 / M phase cells (P <0.05). CONCLUSION: Both 5-aza-2dc and DT alone and in combination can significantly inhibit the proliferation, migration and invasion of PC3 cells at the cell level in vitro, induce cell apoptosis and arrest the cells in G2 / M phase. The combination of both drugs Compared with single drug, the combined effect of 5-aza-2dc and DT has synergistic effect on PC3 cells, which deserves further study.