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目的:探索一种较好的类似于人类糖尿病的模型的方法。方法:选用贵州小香猪,联合应用链脲菌素、四氧嘧啶造模,连续监测血糖浓度;ELISA方法检测造模前后血清胰岛素水平。胰腺HE切片用来评价胰岛状态。结果:造模前,糖尿病模型组和正常对照组的体重、血糖和胰岛素水平均无显著性差异。造模后,与对照组相比,模型组体重显著下降(4.09±0.31 vs.6.42±0.56 kg,P<0.05),血糖显著升高(17.30±1.20 vs.4.30±0.32 mmol/L,P<0.05),胰岛素水平显著下降(0.04±0.02 vs.0.11±0.03μg/L,P<0.05)。HE切片显示模型组胰岛明显萎缩。结论:给小型猪静脉注射60 mg/kg链脲菌素和30 mg/kg四氧嘧啶可成功建立糖尿病模型,该剂量可作为复制猪糖尿病模型的参考标准。
PURPOSE: To explore a better method of modeling similar to human diabetes. Methods: Guizhou small Xiang pigs were used in combination with streptozotocin and alloxan to continuously monitor the blood glucose concentration. Serum insulin levels were measured by ELISA. Pancreatic HE slices were used to assess the islet status. Results: There was no significant difference in body weight, blood glucose and insulin between diabetic model group and normal control group before modeling. Compared with the control group, the body weight of model group decreased significantly (4.09 ± 0.31 vs 6.42 ± 0.56 kg, P <0.05) and the blood glucose increased significantly (17.30 ± 1.20 vs 4.30 ± 0.32 mmol / L, P < 0.05), insulin level decreased significantly (0.04 ± 0.02 vs.0.11 ± 0.03 μg / L, P <0.05). HE slices showed that the islets of the model group were significantly atrophied. CONCLUSIONS: Diabetes mellitus can be successfully established by intravenous injection of 60 mg / kg streptozotocin and 30 mg / kg alloxan in small pigs, and this dose can serve as a reference standard for replicating pig model of diabetes.