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对水稻叶色进行表型分析与基因定位,可为图位克隆该类基因和研究水稻光合系统功能奠定基础。利用甲基磺酸乙酯(ethyl methanesulfonate,EMS)诱变的方法,从籼稻品种“南京11”(简称NJ11)中获得温敏黄化突变体dy1;与野生型相比,dy1在自然环境下,从苗期至成熟期始终表现叶片黄化,透射电镜显示dy1类囊体结构异常;同时株高、分蘖数、结实率等农艺性状也表现出极显著的差异;实验室光照培养箱条件下,dy1苗期在20℃下表现白化,在25℃表现黄化,在30℃下为浅绿的表型;遗传分析表明水稻温敏黄化突变体dy1的表型是由1对隐性基因控制;将突变体与粳稻广亲和品“02428”杂交,构建F2群体,从中选出隐性极端个体,通过基因定位,将控制黄化的基因限定在第1染色体长臂上标记Y-4和Y-35之间的115 kb的区间内,含有16个开放阅读框(ORF),测序发现,dy1中编码尿嘧啶核苷酸激酶的基因LOC_Os01g73450的第4个内含子与第5个外显子交界处存在单碱基替换,拟作为候选基因;且叶绿体合成相关基因表达量显著降低,猜测该基因可能参与了叶绿素合成途径。
The phenotypic analysis and gene mapping of rice leaf color may lay a foundation for cloning these genes and studying the function of rice photosynthesis system. Thermophilic yellowing mutant dy1 was obtained from indica rice “Nanjing 11” (NJ11) by mutagenesis with ethyl methanesulfonate (EMS). Compared with wild type, dy1 Environment, from the seedling stage to the mature stage has always shown the leaf yellowing, transmission electron microscopy showed dy1 thylakoid structural abnormalities; the same time, plant height, tiller number, seed setting rate and other agronomic traits also showed significant differences; laboratory light incubator Under the conditions, the dy1 seedling stage showed whitening at 20 ℃, yellowing at 25 ℃ and light green at 30 ℃. The genetic analysis showed that the phenotype of dy1 in rice was changed from 1 to 2 Sex gene; crossing the mutant with japonica rice broad compatible “02428” to construct F2 population, selecting the most implicit individuals, locating the gene controlling yellowing on the long arm of chromosome 1 The 16 introns of LOC_Os01g73450, a gene encoding uracil nucleotide kinase in dy1, were found to contain 16 open reading frames (ORFs) within the interval of 115 kb between the markers Y-4 and Y-35 with The fifth exon junction exists single base substitution, to be as candidate genes; and chloroplast synthesis related gene table It was significantly reduced speculation that the gene may be involved in chlorophyll biosynthesis.