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目的：研究凝血栓蛋白在体内对新生血管形成的抑制作用，探索新的抗新生血管治疗药物。方法：（１）将基因重组小鼠凝血栓蛋白－１（ｒＴＳＰ１）与内毒素混合制成一种缓释药膜，并将其植入兔角膜层间；（２）在已形成新生血管的兔眼上，球结膜下注射ｒＴＳＰ１，裂隙灯显微镜下观察并记录角膜新生血管的长度及范围，计算其生长面积并进行动态定量分析。结果：植入ｒＴ－ＳＰ１缓释药膜后兔角膜新生血管的形成及生长明显受抑制，球结膜下注射ｒＴＳＰ１，新生血管的生长速度受到一定的抑制，但已形成的血管未发生退化及萎缩。结论：ｒＴＳＰ１能够特异性地抑制体内新生血管的形成，但对已形成的新生血管可能无效，故应早期应用，以预防新生血管的形成。
Objective: To study the inhibitory effect of thrombospondin on neovascularization in vivo and to explore new anti-angiogenic drugs. Methods: (1) Recombinant mouse thrombospondin-1 (rTSP1) was mixed with endotoxin to form a sustained-release membrane and then implanted into the cornea of rabbits. (2) After the formation of neovascularization Rabbit eye, the ball subconjunctival injection rTSP1, slit lamp microscope observation and recording the length and extent of corneal neovascularization, calculate the growth area and dynamic quantitative analysis. Results: The formation and growth of corneal neovascularization in rabbits was significantly inhibited after the rT-SP1 release film was implanted. The growth velocity of corneal neovascularization was inhibited by subconjunctival injection of rTSP1. However, the growth of neovascularization was inhibited to a certain extent, but no degenerated and atrophic . Conclusion: rTSP1 can specifically inhibit the formation of neovascularization in vivo, but may not be effective in the formation of new blood vessels. Therefore, rTSP1 should be used early to prevent the formation of new blood vessels.