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目的研究旋毛虫对三硝基苯磺酸(TNBS)诱导的实验性小鼠肠炎模型的影响及其免疫作用机制。方法观察感染和未感染旋毛虫小鼠于TNBS诱导肠炎后3 d及7 d不同指标的变化,包括小鼠生存率、疾病活动指数(DM)、结肠大体损伤和病理损伤评分、炎症指标髓过氧化物酶(MPO)活性检测,结肠细胞因子IFN-γ和IL-4 mRNA的表达量分析。结果预先感染旋毛虫后诱导TNBS模型组小鼠在造模后3 d及7 d与单纯模型组相比小鼠生存率升高(P<0.05),DAI、结肠大体损伤和病理损伤评分及MPO活性下降(P<0.05),结肠中IFN-γmRNA的表达量下调(P<0.05),而IL-4 mRNA的表达量增加(P<0.05)。结论旋毛虫对TNBS诱导的实验性小鼠肠炎具有良好的干预作用,其免疫作用机制可能是通过下调炎症性肠病过度的T_H1型免疫反应、上调T_H2型免疫反应而实现的。
Objective To study the effects of Trichinella spiralis on TNBS-induced murine enteritis and its immunological mechanism. Methods The changes of different indexes of infected and uninfected Trichinella were observed on the 3rd and 7th day after TNBS-induced enteritis, including the survival rate of mice, disease activity index (DM), general colonic injury and pathological damage score, The activity of oxidase (MPO) and the expression of IFN-γ and IL-4 mRNA in colon were analyzed. Results Compared with the model group, the survival rate of mice in TNBS model group was significantly increased (P <0.05) at 3 days and 7 days after induction of Trichinella spiralis infection. The scores of DAI, gross colonic injury and pathological damage score and MPO (P <0.05). The expression of IFN-γmRNA in colon decreased (P <0.05), and the expression of IL-4 mRNA increased (P <0.05). Conclusion Trichinella spiralis has a good effect on the TNBS-induced experimental mouse enteritis, and the immunological mechanism may be through down-regulation of T_H1-type immune response and up-regulation of T_H2-type immune response in inflammatory bowel disease.