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目的:建立舒筋定痛片中大黄素和大黄酚的高效液相含量测定方法。方法:采用Apollo C18柱(4.6 mm×250 mm,5μm),以甲醇-0.1%磷酸溶液(85∶15)为流动相,检测波长为290 nm。结果:大黄素在0.0194~0.0582μg进样量范围内线性关系良好,r=0.9999,平均回收率为96.74%,RSD为1.99%;大黄酚在0.0551~0.165μg进样量范围内线性关系良好,r=0.9999,平均回收率为99.02%,RSD为2.48%。结论:该方法简单,重复性好,可用于舒筋定痛片的质量控制。
Objective: To establish a HPLC method for the determination of emodin and chrysophanol in Shujin Dingtong Tablets. Methods: Apollo C18 column (4.6 mm × 250 mm, 5 μm) was used with methanol-0.1% phosphoric acid solution (85:15) as the mobile phase. The detection wavelength was 290 nm. Results: The emodin showed a good linearity in the range of 0.0194 ~ 0.0582μg, with an average recovery of 96.74% and a RSD of 1.99% (r = 0.9999). There was a good linear relationship between emodin and 0.0551 ~ 0.165μg injection volume, r = 0.9999, the average recovery was 99.02%, RSD was 2.48%. Conclusion: The method is simple and reproducible, and can be used for quality control of Shujintongtong Tablet.